Triple screening of gynecology cancers using cervical DNA methylation

Poster presented at European Gynaecological Oncological Congress 2015

Yu-Ping Laio, Po-Hsuan Su, Rui-Lan Huang, Hui-Chen Wang, Yu-Chih Chen, Hung-Cheng Lai

Introduction: Epigenetic changes play an important role in cancer development. Our previous research on the epigenomics of cervical cancer using genome-wide approaches identified 14 candidate genes that were hypermethylated in cervical cancer tissues. Most of these genes are transcription factors and development-related genes that are common in the development of various cancers. Recent researchers demonstrated that gene mutations in cervical scrapings can reflect the status of endometrial and ovarian cancer tissues.

Aim: We hypothesized that DNA methylation at cervical scrapings could detect the presence of endometrial/ovarian cancers.

Material & Methods: We tested the methylation status of these 14 genes in endometrial and ovarian cancer tissues. Genes hypermethylated in cancer tissues were selected for further testing using cervical scrapings from 19 endometrial or 27 ovarian cancer patients and 25 controls. The evaluation of the clinical performance characteristics of DNA methylation, including sensitive, specificity, positive predictive value, and negative predictive value were calculated.

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